Targeting VEGF with LNA-stabilized G-rich oligonucleotide for efficient breast cancer inhibition

• DOI: 10.1039/c5cc02756j
• Publication Date: 2015-05-07T16:00:50Z
• Authors: Stacey L. Edwards; Vasanthanathan Po...; Jagat R. Kanwar; Kislay Roy; Kristine M. Hillman; Neerati Prasad; Rikke Leth-Larsen; Michael Petersen; Maja Marušič; Janez Plavec; Jesper Wengel; Rakesh N. Veedu
• Keywords: Vascular Endothelial Growth Factor A; 0301 basic medicine; Aptamers, Nucleotide/chemistry; Guanine; 1503 Catalysis; 2504 Electronic; Nude; 2506 Metals and Alloys; Oligonucleotides; Mice, Nude; 2503 Ceramics and Composites; Cell Movement/drug effects; Antineoplastic Agents; Breast Neoplasms; Human Umbilical Vein Endothelial Cells/drug effects; Molecular Dynamics Simulation; Aptamers; Cell Line; Coatings and Films; Cell Proliferation/drug effects; Mice; 03 medical and health sciences; Breast Neoplasms/drug therapy; Cell Movement; Cell Line, Tumor; Antineoplastic Agents/chemistry; Human Umbilical Vein Endothelial Cells; Animals; Humans; Optical and Magnetic Materials; Oligonucleotides/chemistry; 2505 Materials Chemistry; Cell Proliferation; Multidisciplinary; Tumor; Nucleotide/chemistry; Vascular Endothelial Growth Factor A/metabolism; Aptamers, Nucleotide; 1600 Chemistry; Tumor Burden; 3. Good health; Molecular Docking Simulation; Chemistry; 2508 Surfaces; Female; Tumor Burden/drug effects

In this study, we investigated the efficacy of an LNA (locked nucleic acid)-modified DNA aptamer named RNV66 targeting VEGF against various breast cancer cell lines.