A new ratiometric fluorescent probe for rapid, sensitive and selective detection of endogenous hydrogen sulfide in mitochondria

• DOI: 10.1039/c5cc09267a
• Publication Date: 2016-01-19T10:38:47Z
• Authors: Xiao Feng; Tao Zhang; Jin-Ting Liu; Jun-Ying Miao; Bao-Xiang Zhao
• Keywords: Limit of Detection; Hydrogen Sulfide; 01 natural sciences; Fluorescent Dyes; Mitochondria; 0104 chemical sciences

We have developed a new ratiometric fluorescent probe composed of coumarin-merocyanine dyad based on the FRET mechanism. The showed clear dual-emission signal changes in blue and red spectral windows upon addition H2S dose dependent manner under single wavelength excitation. targeted mitochondria with high selectivity sensitivity toward H2S.