Potent inhibitory effect of silibinin from milk thistle on skin inflammation stimuli by 12-O-tetradecanoylphorbol-13-acetate

• DOI: 10.1039/c5fo00899a
• Publication Date: 2015-08-24T14:30:52Z
• Authors: Wenfeng Liu; Yonglian Li; Xi Zheng; Kun Zhang; Zhiyun Du
• Keywords: Silibinin; Silybum marianum; 12-O-Tetradecanoylphorbol-13-acetate; Milk Thistle; IκB kinase

Silibinin, a major polyphenol in milk thistle, has been reported to have multiple pharmacological activities; therefore, there is an urgent need well understand how silibinin works on inflammation-associated skin diseases. We herein designed 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated inflammation test its inhibitory effects. It was demonstrated that silibinin, applied topically onto mouse ears following TPA stimulation, effectively down-regulated the expressions of TPA-induced interleukin-1β (IL-1β), interleukin-6 (IL-6), necrosis factor-alpha (TNF-α) and cyclooxygenase-2 (COX-2) dose-dependent manner. Further mechanistic investigations indicated suppressed expression IκB kinase (IKK) by inhibiting phosphoinositide 3-kinase/protein B (PI3K/Akt) signaling pathway, thereby suppressing TPA-stimulated nuclear factor-κB (NF-κB) activation. Promisingly, used for transdermal application, may be potent naturally occurring anti-inflammatory agent prevention