Sub-7-second genotyping of single-nucleotide polymorphism by high-resolution melting curve analysis on a thermal digital microfluidic device

• DOI: 10.1039/c5lc01533b
• Publication Date: 2016-01-05T14:38:25Z
• Authors: Tianlan Chen; Yanwei Jia; Cheng Dong; Jie Gao; Pui-In Mak; Rui P. Martins
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Time Factors; Base Sequence; Genotyping Techniques; 13. Climate action; Lab-On-A-Chip Devices; Transition Temperature; DNA; Nucleic Acid Denaturation; Electrodes; Polymorphism, Single Nucleotide

A novel thermal digital microfluidic (T-DMF) device enables precise thermal modulation and pipelined measurement of multiple samples. Ultrafast DNA melting curve analysis is achieved in less than 7 seconds, with the resolution adequate for single-nucleotide discrimination.