Mesoporous bioactive glass nanolayer-functionalized 3D-printed scaffolds for accelerating osteogenesis and angiogenesis

• DOI: 10.1039/c5nr05421d
• Publication Date: 2015-10-19T11:04:15Z
• Authors: Yali Zhang; Lunguo Xia; Dong Zhai; Mengchao Shi; Yongxiang Luo; Chun Feng; Bing Fang; Jingbo Yin; Jiang Chang; Chengtie Wu
• Keywords: Bioceramic; Bioactive Glass; RUNX2

The hierarchical microstructure, surface and interface of biomaterials are important factors influencing their bioactivity. Porous bioceramic scaffolds have been widely used for bone tissue engineering by optimizing chemical composition large-pore structure. However, the struts in often ignored. aim this study is to incorporate pores bioactive components into constructing nanopores elements on further improve bone-forming activity. Mesoporous glass (MBG) modified β-tricalcium phosphate (MBG-β-TCP) with a pore structure functional strut (∼100 nm MBG nanolayer) were successfully prepared via 3D printing spin coating. compressive strength apatite-mineralization ability MBG-β-TCP significantly enhanced as compared β-TCP without nanolayer. attachment, viability, alkaline phosphatase (ALP) activity, osteogenic gene expression (Runx2, BMP2, OPN Col I) protein (OPN, I, VEGF, HIF-1α) rabbit marrow stromal cells (rBMSCs) well viability angiogenic (VEGF human umbilical vein endothelial (HUVECs) upregulated conventional (BG)-modified (BG-β-TCP) pure scaffolds. Furthermore, formation new vivo BG-β-TCP results suggest that application nanolayer modify 3D-printed offers strategy construct hierarchically porous improved physicochemical biological properties, such mechanical osteogenesis, angiogenesis applications, which incorporation nanostructures scaffold synergistically play key role formation.