Dual physical dynamic bond-based injectable and biodegradable hydrogel for tissue regeneration

• DOI: 10.1039/c5tb02254a
• Publication Date: 2016-01-05T14:12:18Z
• Authors: Xiaochu Ding; Jin Gao; Hassan Awada; Yadong Wang

A biodegradable and injectable hydrogel was designed using dual physical dynamic bonds based on host-guest chemistry electrostatic interactions to build up the network structure. The material synthesized by simultaneously coupling mono-carboxylic acid terminated poly(ethylene glycol) arginine aspartate diglyceride) yield a mPEG-grafted argininylaspartate (mPEG-g-PEAD). When mixing this polymer with α-cyclodextrin natural polyanion (heparin), supramolecular formed in quick gelation shear thinning properties. vitro cytotoxicity evaluated primary baboon arterial smooth muscle cells (BaSMCs) results showed that cell membrane integrity, viability metabolism were not compromised synthetic polycation at concentrations as high 10 mg mL-1, 1000-fold lower toxicity than commercial PEI. biocompatibility of as-made also BaSMCs. Neither nor components altered behavior assays. Fibroblast growth factor 2 incorporated into sustainably released nearly stable rate 16 days without initial burst release, suggesting potential applications wound healing ischemic tissue regeneration, among others.