Vibration-Induced-Emission (VIE) for imaging amyloid β fibrils

• DOI: 10.1039/c6fd00156d
• Publication Date: 2016-08-01T10:32:54Z
• Authors: Wei-Tao Dou; Wei Chen; Xiao-Peng He; Jianhua Su; He Tian
• Keywords: Amyloid (mycology); Fluorescence-lifetime imaging microscopy

This paper discusses the use of N,N'-disubstituted-dihydrodibenzo[a,c]phenazines with typical Vibration-Induced-Emission (VIE) properties for imaging amyloid β (Aβ) fibrils, which are a signature neurological disorders such as Alzheimer's disease. A water-soluble VIEgen red fluorescence emission shows pronounced, blue-shifted Aβ peptide monomers and fibrils. The enhancement in blue can be ascribed to restriction molecular vibration by selectively binding Aβ. We determine an increasing blue-to-red ratio both concentration fibrogenesis time Aβ, thereby enabling ratiometric detection its different morphological forms. Importantly, was proven suitable small plaques hippocampus transgenic mouse brain (five months old), emissions well overlapped on research offers new rationale design VIE probes biological applications.