Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging

• DOI: 10.1039/c6mt00243a
• Publication Date: 2016-11-23T12:46:56Z
• Authors: J. L. Wedding; H. H. Harris; C. A. Bader; S. E. Plush; R. Mak; M. Massi; D. A. Brooks; B. Lai; S. Vogt; M. V. Werrett; P. V. Simpson; B. W. Skelton; S. Stagni
• Keywords: Fluorescence-lifetime imaging microscopy

Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline L 5-(4-iodophenyl)tetrazolato, 22Rv1 cells. The rhenium showed no signs ancillary ligand dissociation, a conclusion based on data obtained via aligning iodine distributions. A diffuse reticular localisation detected for nuclear/perinuclear region cells, by either optical or techniques. also that disrupted homeostasis some biologically relevant elements, such as chlorine, potassium zinc.