Oxidase-mimicking activity of ultrathin MnO2nanosheets in colorimetric assay of acetylcholinesterase activity

• DOI: 10.1039/c6nr08473g
• Publication Date: 2017-01-05T08:23:57Z
• Authors: Xu Yan; Yang Song; Xiaoli Wu; Chengzhou Zhu; Xingguang Su; Dan Du; Yuehe Lin
• Keywords: Paraoxon; Acetylthiocholine; Absorbance; Horseradish peroxidase; Colorimetry; Choline oxidase

In the present study, a novel colorimetric sensing platform was constructed for quantitative detection of acetylcholinesterase (AChE) activity and its inhibitor. Manganese dioxide (MnO2) nanosheets as an oxidase-mimicking nanomaterial could directly oxidize 3,3',5,5'-tetramethylbenzidine (TMB) into oxTMB without need horseradish peroxidase H2O2. When AChE introduced, acetylthiocholine be catalytically hydrolyzed to produce thiocholine, which easily triggers decomposition MnO2 nanosheets, causing decrease solution absorbance. Owing inhibition effect organophosphorus pesticides, enzymatic suppressed, preventing resulting in increase Under optimal conditions, shows sensitive responses paraoxon range 0.1-15 mU mL-1 0.001-0.1 μg mL-1, respectively. The limits reached 35 μU 1.0 ng Furthermore, MnO2-TMB has been used fabricate test strips rapid convenient visual inhibitor with highly promising performance.