A ratiometric fluorescence probe for imaging sulfur dioxide derivatives in the mitochondria of living cells

• DOI: 10.1039/c6ob02779b
• Publication Date: 2017-01-05T18:54:01Z
• Authors: Yongfei Wang; Qingtao Meng; Run Zhang; Hongmin Jia; Xuehu Zhang; Zhiqiang Zhang
• Keywords: 1303 Biochemistry; Molecular Structure; Optical Imaging; MCF-7 Cells; Humans; Sulfur Dioxide; 1606 Physical and Theoretical Chemistry; 540; 01 natural sciences; 1605 Organic Chemistry; Fluorescent Dyes; Mitochondria; 0104 chemical sciences

Although sulfur dioxide (SO2) plays an essential role in several physiological processes, monitoring of intracellular SO2 at subcellular levels remains challenging due to the lack rapid and sensitive methods for its quantification a 100% aqueous solution. Herein, new hemicyanine dyes-based fluorescence probe, NBD-Id, was designed synthesized detection derivatives pure solution living cells. By virtue specific 1,4-addition reaction SO32-HSO3- polymethine chain hemicyanine, significant changes absorption emission spectra were observed less than 20 seconds. The ratiometric (F467/F593) then obtained with high sensitivity (detection limit 3.6 nM). It noted that NBD-Id has response towards without interference from other anions biomolecules. Intracellular imaging indicated is cell membrane permeable mainly distributed within mitochondria. Therefore, mitochondria MCF-7 cells successfully demonstrated.