Quantum dot-based sensitive detection of disease specific exosome in serum

• DOI: 10.1039/c7an00672a
• Publication Date: 2017-05-04T14:24:34Z
• Authors: Kseniia Boriachek; Md. Nazmul Islam; Vinod Gopalan; Alfred K. Lam; Nam-Trung Nguyen; Muhammad J. A. Sh...
• Keywords: Exosome

Tumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific using quantum dots signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by generic tetraspanin antibody (e.g., CD9 or CD63) followed identification cancer-related. Here, used CdSe dot (CdSeQD) functionalised-biotinylated HER-2 FAM134B antibodies breast colon markers. After washing purification steps, acid dissolution CdSeQDs anodic quantification Cd2+ were carried out at bare glassy carbon working electrode. This method enabled sensitive 100 per μL with relative standard deviation (%RSD) <5.5% in cell lines small cohort serum samples (n = 9) collected from patients colorectal adenocarcinoma. We believe that our approach could potentially represent an effective bioassay clinical samples.