Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe

• DOI: 10.1039/c8cc02135j
• Publication Date: 2018-06-14T07:24:13Z
• Authors: Marco P. Monopoli; Andrea Zendrini; Dan Wu; Shane Cheung; Gonzalo Sampedro; Brendan Ffrench; John Nolan; Olga Piskareva; Raymond L. Stalings; Serena Ducoli; Paolo Bergese; Donal F. O’Shea
• Keywords: Exosome; Extracellular Vesicles

The recognition of the biological, diagnostic and medical importance exosomes has given rise to an urgent need for efficient labelling these extracellular vesicles in ways that do not alter their inherent characteristics. We report first time endogenous method NIR-fluorescent labelled using amphiphilic probe without immunolabelling or synthetic chromatographic manipulation exosomes. Comparative analyses unlabelled with NTA, AFM, flow cytometry immunoblot analysis all show a high degree similarity. Spectroscopic fluorescence imaging confirmed ability visualise purified NIR-exosomes.