Plumbagin induces ROS-mediated apoptosis and cell cycle arrest and inhibits EMT in human cervical carcinoma cells

• DOI: 10.1039/c8ra05339a
• Publication Date: 2018-09-17T07:24:26Z
• Authors: Ankita Jaiswal; Akash Sabarwal; Jai Prakash Naray...; Rana P. Singh
• Keywords: Plumbagin

Plumbagin, an important phytochemical from the roots of medicinal plant Plumbago zeylanica L. has shown many biological activities. The this have been in use Indian system medicine for more than twenty five centuries treatments various ailments. It anticancer activities, however, and anti-metastatic effects plumbagin are largely unknown against cervical cancer cells. Herein, we investigated molecular alterations associated with plumbagin-mediated inhibition growth, survival epithelial to mesenchymal transition human SiHa HeLa Plumbagin (1-4 μM) caused a significant decrease cell viability increased death Hela cells after 24 48 h. also strong G2/M S-G2/M phase cycle arrest cells, respectively which was accompanied by expression cyclin CDK levels. levels both mRNAs proteins B1, A E2 1 2 decreased strongly induced apoptosis along ratio Bax : Bcl2 cleavage caspase 3, 9, PARP. increase reactive oxygen species generation mediated as it attenuated pre-treatment N-acetyl cysteine. Additionally, report first time that possesses effect at non-cytotoxic doses modulation MMP-2, E-cadherin, N-cadherin, β-catenin vimentin. Taken together, our findings suggest