Near-infrared fluorescent probes based on TBET and FRET rhodamine acceptors with different pKa values for sensitive ratiometric visualization of pH changes in live cells

• DOI: 10.1039/c8tb01524d
• Publication Date: 2018-11-16T16:10:50Z
• Authors: Jianbo Wang; Shuai Xia; Jianheng Bi; Yibin Zhang; Mingxi Fang; Rudy L. Luck; Yanbo Zeng; Tzu-Ho Chen; Hsien-Ming Lee; Haiying Liu
• Keywords: Rhodamines; Fluorescence Resonance Energy Transfer; Humans; Hydrogen-Ion Concentration; Lysosomes; 01 natural sciences; Fluorescent Dyes; HeLa Cells; 0104 chemical sciences

Three near-infrared ratiometric fluorescent probes with different pKa values were synthesized to achieve sensitive ratiometric visualization of pH variations in lysosomes.