In vitroquantification of botulinum neurotoxin type A1 using immobilized nerve cell-mimicking nanoreactors in a microfluidic platform

• DOI: 10.1039/c9an00817a
• Publication Date: 2019-08-13T08:45:43Z
• Authors: Oliver G. Weingart; Klaus Eyer; Christian Lüchten...; Timo Sachsenheimer; Britta Brügger; Marc van Oostrum; Bernd Wollscheid; Petra S. Dittrich; Martin J. Loessner
• Keywords: Neurons; 0303 health sciences; Swine; Serum Albumin, Human; Biosensing Techniques; Cells, Immobilized; In Vitro Techniques; Microfluidic Analytical Techniques; Surface Plasmon Resonance; Nanostructures; 3. Good health; 03 medical and health sciences; Biomimetic Materials; Lab-On-A-Chip Devices; Liposomes; Animals; Botulinum Toxins, Type A; Drugs, Chinese Herbal; Protein Binding

Herein we report anin vitroapproach to selectively quantify physiologically active BoNT/A only. This technology is based on the use of nerve cell-mimicking nanoreactors in combination with microfluidics.