A hydroxylamine probe for profiling S-acylated fatty acids on proteins

• DOI: 10.1039/c9cc05989j
• Publication Date: 2019-08-29T13:23:34Z
• Authors: Janine Schulte-Zw...; Mridula Dwivedi; Andreas Brockmeyer; Petra Janning; Roland Winter; Gemma Triola
• Keywords: 0301 basic medicine; Molecular Structure; Acylation; Fatty Acids; Hydroxylamine; S-acylome; Proto-Oncogene Proteins p21(ras); Mice; 03 medical and health sciences; HEK293 Cells; Cell Line, Tumor; Animals; Humans; Fluorescent Dyes; HeLa Cells

An hydroxylamine probe combined with mass spectrometry analysis enable accurate identification of fatty acids in S-acylated proteins.