Pyrococcus furiosus Argonaute-mediated nucleic acid detection

• DOI: 10.1039/c9cc07339f
• Publication Date: 2019-10-02T03:03:33Z
• Authors: Ruyi He; Longyu Wang; Fei Wang; Wenqiang Li; Yang Liu; Aitao Li; Yuan Wang; Wuxiang Mao; Chao Zhai; Lixin Ma
• Keywords: Pyrococcus furiosus; Argonaute; Cleave; Cleavage (geology); Molecular beacon

<italic>Pf</italic>Ago-mediated Nucleic acid Detection (PAND) distinguishes single-nucleotide mutants and accomplishes multiplexed detection by a second round of cleavage.