Engineering bromodomains with a photoactive amino acid by engaging ‘Privileged’ tRNA synthetases

• DOI: 10.1039/c9cc09891g
• Publication Date: 2020-02-19T15:03:52Z
• Authors: Shana Wagner; Babu Sudhamalla; Philip Mannes; Sushma Sappa; Sam Kavoosi; Debasis Dey; Sinan Wang; Kabirul Islam
• Keywords: Amino Acyl-tRNA Synthetases; 0301 basic medicine; 03 medical and health sciences; Cross-Linking Reagents; Molecular Structure; 13. Climate action; Phenylalanine; Amino Acids; Photochemical Processes; Protein Engineering; 7. Clean energy; 01 natural sciences; 0104 chemical sciences

An improved chemical synthesis, site-specific incorporation and enhanced photo-crosslinking ability of tmdF have been demonstrated in the context of protein–protein interactions.