Oleanolic acid enhances tight junctions and ameliorates inflammation in Salmonella typhimurium-induced diarrhea in mice via the TLR4/NF-κB and MAPK pathway

• DOI: 10.1039/c9fo01718f
• Publication Date: 2019-11-13T02:23:34Z
• Authors: Na Dong; Chenyu Xue; Lei Zhang; Tingting Zhang; Chensi Wang; Chongpeng Bi; Anshan Shan
• Keywords: Diarrhea; Male; Salmonella typhimurium; 0301 basic medicine; Mice, Inbred BALB C; Tight Junction Proteins; NF-kappa B; Tight Junctions; 3. Good health; Toll-Like Receptor 4; Mice; 03 medical and health sciences; Cyclooxygenase 2; Salmonella Infections; Animals; Cytokines; Oleanolic Acid

Salmonella typhimurium (S.T) is a common cause of acute, self-limiting food-borne diarrhea with severe intestinal inflammation and barrier damage. Oleanolic acid (OA), isolated from almost 2000 plant species, has been shown to have anti-inflammatory roles. The purpose this study was investigate the potential protective effects OA on S.T-induced enteritis elucidate its mechanisms. A total eighty BALB/c mice (4-week-old) were randomly divided into control group (no S.T, no OA), S.T (S.T only), + plus 100 mg kg-1 OA) (100 only). Compared group, administration significantly reduced clinical symptoms weight loss, severity structural damage alleviated, which confirmed by decrease in index (DI) jejunal histological In addition, infected jejunum, maintained expression localization occludin, claudin-1 ZO-1 protect barrier, thereby maintaining integrity gut barrier. Finally, treatment not only levels COX-2 iNOS but also inhibited secretion pro-inflammatory cytokines, such as IL-1β, IL-6 TNF-α. Furthermore, western blotting results showed that IκB phosphorylation degradation tissues nuclear translocation p65, decreased level TLR4 activation MAPK pathway. To summarise, can maintain tight junction prevent caused S.T. well reduce through NF-κB signaling pathways.