Cytotoxic and antimicrobial indole alkaloids from an endophytic fungusChaetomiumsp. SYP-F7950 ofPanax notoginseng

• DOI: 10.1039/c9ra04747f
• Publication Date: 2019-09-12T12:03:52Z
• Authors: Fei Peng; Shao-Yang Hou; Tian-Yuan Zhang; Ying-Ying Wu; Meng-Yue Zhang; Xi-Meng Yan; Ming-Yu Xia; Yi-Xuan Zhang
• Keywords: Panax notoginseng

Two new compounds chetoseminudin F (1) and G (2) together with eleven known were isolated from the solid fermentation products of endophytic fungus Chaetomium sp. SYP-F7950. The structures elucidated by extensive spectroscopic analyses, including 1D 2D NMR, HRFABMS experiments. absolute configurations determined comparing electronic circular dichroism (ECD) spectrum those reported references. A plausible biogenetic pathway for 1-6 9-13 was proposed. These isolates also evaluated their antimicrobial antitumor activity, revealing that displayed more potent cytotoxicity against MDA-MB-231 cells an IC50 value 26.49 μmol L-1 than common chemotherapeutic agent (paclitaxel). In assay, 6, 9, 11 12 had strong antibacterial activity Staphylococcus aureus, Bacillus subtilis, Enterococcus faecium antifungal Candida albicans minimum inhibitory concentration (MIC) values ranging 0.12 to 9.6 μg mL-1; meanwhile 8, 9 exhibited 2.75-8.68 tumor cell lines A549 MDA-MB-231. addition, morphological observation showed treatment increased mean length B. subtilis 1.6 1.8-fold. silico molecular docking applied study binding interactions between active sites filamentous temperature-sensitive protein Z (FtsZ) subtilis. Compounds low energies, H-bond FtsZ. On basis activities, cellular phenotype studies, are considered be a promising inhibitor