Ti3C2Tx MXene-derived TiO2/C-QDs as oxidase mimics for the efficient diagnosis of glutathione in human serum

• DOI: 10.1039/c9tb02478f
• Publication Date: 2020-01-02T12:46:03Z
• Authors: Zhaoyong Jin; Gengfang Xu; Yusheng Niu; Xiaoteng Ding; Yaqian Han; Wenhan Kong; Yanfeng Fang; Haitao Niu; Yuanhong Xu
• Keywords: Oxidizing agent; Absorbance; Carbon fibers

Nanozyme-based colorimetry was suggested to be a rapid method for biomarker (e.g. glutathione) detection, but this suffers from lack of efficiency and low-toxicity nanozymes till now. Herein, quantum dots TiO2 loaded on carbon (TiO2/C-QDs) oxidase-like were prepared via hydrothermal treatment tiny few-layered Ti3C2Tx MXene nanosheets, which possess abundant thermodynamic metastable Ti atoms margins as raw materials the preparation TiO2/C-QDs. The oxygen vacancy in surface matrix can facilitate O2 adsorption solution generate reactive species (ROS), thereby quickly oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) its oxidized form (TMBox) absence H2O2. After adding glutathione (GSH), TMBox able restored TMB, resulted corresponding decrease UV-vis absorbance value at 652 nm. Furthermore, assay possesses good selectivity, excellent specificity high sensitivity (limit detection: 0.2 μM), made it possible efficiently detect GSH complex biological samples such human serum.