Lipidomic atlas of mammalian cell membranes reveals hierarchical variation induced by culture conditions, subcellular membranes, and cell lineages

• DOI: 10.1039/d0sm00404a
• Publication Date: 2020-05-13T17:36:43Z
• Authors: Jessica L. Symons; Kwang-Jin Cho; Jeffrey T. Chang; Guangwei Du; M. Neal Waxham; John F. Hancock; Ilya Levental; Kandice R. Levental
• Keywords: Organelle; Lipidome; Compartmentalization (fire protection); Cell type; Cell membrane; Cell Physiology

Lipid membranes are ubiquitous biological organizers, required for structural and functional compartmentalization of the cell sub-cellular organelles. Membranes in living cells compositionally complex, comprising hundreds dynamically regulated, distinct lipid species. Cellular physiology requires tight regulation these lipidomic profiles to achieve proper membrane functionality. While some general features tissue- organelle-specific complements have been identified, less is known about detailed variations caused by cell-intrinsic or extrinsic factors. Here, we use shotgun lipidomics report detailed, comprehensive lipidomes a variety cultured primary mammalian preparations identify trends sources variation. Unbiased principle component analysis (PCA) shows clear separation between cells, with erythrocytes, synaptic membranes, other tissue sharply diverging from all lines also one other. Most broadly, were distinguished their paucity polyunsaturated lipids. Cultured comparatively similar another, although detected clear, highly reproducible signatures individual plasma (PM) isolations thereof. These measurements begin establish atlas tissues, identifying major observations will allow investigation significance various contexts.