Sequential macrophage transition facilitates endogenous bone regeneration induced by Zn-doped porous microcrystalline bioactive glass

• DOI: 10.1039/d0tb02884c
• Publication Date: 2021-03-06T06:33:30Z
• Authors: Xuan Bai; Wenjuan Liu; Laijun Xu; Qing Ye; Huasi Zhou; Camilla Berg; He Yuan; Jiyao Li; Wei Xia
• Keywords: Proinflammatory cytokine

Macrophages play an important role in the immune microenvironment during bone healing, and sequential macrophage phenotypic transition could achieve superior osteogenic outcomes. Microcrystalline bioactive glasses (MCBGs) with osteoimmunomodulatory effects show potential tissue regeneration. Zinc (Zn) has been approved to coordinate innate adaptive immunity. Therefore, this study, different amounts of ZnO were incorporated into microcrystalline glass improve its immunomodulatory ability. The effect Zn-MCBG ionic extracts on was studied, 5Zn-MCBG orchestrate M1-to-M2 promote expression proinflammatory anti-inflammatory genes cytokine induce human marrow stromal cells (hBMSCs) differentiation vitro. Macroporous scaffolds containing mesopores fabricated showed good cell adhesion feasible apatite formation when immersed SBF Furthermore, a rat calvarial defect model used confirm that scaffold modulate create desirable osteogenesis vivo.