Orthogonal coiled coils enable rapid covalent labelling of two distinct membrane proteins with peptide nucleic acid barcodes

• DOI: 10.1039/d1cb00126d
• Publication Date: 2021-07-16T09:43:46Z
• Authors: Georgina C. Gavins; Katharina Gröger; Marc Reimann; Michael D. Bartos...; Sebastian Bultmann; Oliver Seitz
• Keywords: Bioconjugation; Template; Peptide nucleic acid

A pair of orthogonal coiled coils templates highly specific live cell bioconjugation two different proteins. PNA tagging and hybridisation with fluorophore–DNA reporters enables rapid dual receptor internalisation analysis EGFR ErbB2.