Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library

• DOI: 10.1039/d1cb00147g
• Publication Date: 2021-08-20T14:21:18Z
• Authors: Naoki Miyamoto; Young-Hyun Go; Larissa Miasiro C...; Haw-Young Kwon; Heon Seok Kim; Xuezhi Bi; Young Hyun Yu; Beomsue Kim; Hyung-Ho Ha; Nam-Young Kang; Seong-Wook Yun; Jin-Soo Kim; Hyuk-Jin Cha; Young-Tae Chang
• Keywords: Identification; Matrix (chemical analysis)

CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the cell selectivity mechanism of has not been fully understood. Here, we report ALDH2 ABCB1 as molecular targets CDy1, elucidated by live-cell affinity-matrix ABC transporter CRISPRa library screening. The two unique orthogonal mechanisms provide potential multi-demensional cellular distinction specific types.