The C-terminal loop of Arabidopsis thaliana guanosine deaminase is essential to catalysis

• DOI: 10.1039/d1cc03042f
• Publication Date: 2021-08-14T00:44:40Z
• Authors: Qian Jia; Hui Zeng; Huanxi Li; Nan Xiao; Jing Tang; Shangfang Gao; Jinbing Zhang; Wei Xie
• Keywords: Models, Molecular; 0303 health sciences; 03 medical and health sciences; Arabidopsis; Biocatalysis; Nucleoside Deaminases

We report the crystal structures of enzyme GSDA in various forms at high resolutions, which reveal its recognition and catalytic mechanism may potentially aid development novel deaminase-based gene-editing tools.