Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice

• DOI: 10.1039/d1fo02288a
• Publication Date: 2021-09-20T10:25:33Z
• Authors: Shengjie Li; Aoxiang Zhuge; Kaicen Wang; Longxian Lv; Xiaoyuan Bian; Liya Yang; Jiafeng Xia; Xianwan Jiang; Wenrui Wu; Shuting Wang; Qiangqiang Wang; Lanjuan Li
• Keywords: Ketogenic Diet

Inflammatory bowel disease (IBD) is an idiopathic inflammatory with a high incidence. Multiple factors including dietary composition contribute to its occurrence. Recently, ketogenic diet which consists of proportion fat and low carbohydrates has gained great popularity. Our study aimed explore the effect on IBD potential mechanisms. C57BL/6 mice were given or control for month was induced by 2% DSS in drinking water last week. Gut histology, cytokines chemokines, gut microbiota metabolism assessed. Ketogenic substantially worsened colitis, terms higher body weight loss, DAI scores histological as well colon length shortening. Levels serum chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF IL-10) significantly up-regulated treated DSS. Increased intestinal permeability decreased expressions epithelial barrier associated genes observed due administration. Pretreatment alters bacterial abundance, increasing pathogenic taxa such Proteobacteria, Enterobacteriaceae, Helicobacter Escherichia-Shigella decreasing beneficial Erysipelotrichaceae. also modified metabolism, metabolites bile secretion ouabain, taurochenodeoxycholic acid, quinine, cholic acid glycocholic biosynthesis unsaturated fatty acids stearic arachidic erucic docosanoic acid. These results suggest that aggravates DSS-induced colitis systemic inflammation, disrupting barrier, from modulated metabolism.