Identification and in situ removal of an inhibitory intermediate to develop an efficient phytosterol bioconversion process using a cyclodextrin-resting cell system

• DOI: 10.1039/d1ra02774c
• Publication Date: 2021-07-15T10:54:34Z
• Authors: Da Wang; Jian Zhang; Dan-Dan Cao; Xuedong Wang; Dongzhi Wei
• Keywords: Bioconversion; Phytosterol; Identification

We identified an inhibitory intermediate, 4-ene-3-keto steroids, that limits the bioconversion rate and provided a solution based on resin adsorption for improving 9α-OH-AD production efficiency in commercial-scale process.