Chemical synthesis of stimuli-responsive guide RNA for conditional control of CRISPR-Cas9 gene editing

• DOI: 10.1039/d1sc01194d
• Publication Date: 2021-05-19T12:16:30Z
• Authors: Chunmei Gu; Lu Xiao; Jiachen Shang; Xiao Xu; Luo He; Yu Xiang
• Keywords: Chemistry; 0303 health sciences; 03 medical and health sciences

Conditional control of CRISPR-Cas9 activity by reactive oxygen species and visible light is achieved using stimuli-responsive guide RNA synthesized by a general method based on RNA 2′-O-methylribonucleotide phosphorothioate.