Digestion characteristics of quinoa, barley and mungbean proteins and the effects of their simulated gastrointestinal digests on CCK secretion in enteroendocrine STC-1 cells

• DOI: 10.1039/d2fo00243d
• Publication Date: 2022-05-02T06:02:20Z
• Authors: Hongdong Song; Qiuyun Fu; Kai Huang; Zhiying Zou; Limin Chen; Hulin Chen; Shaocheng Ge; Jing Wang; Xiao Guan
• Keywords: Enteroendocrine cell; Digestion; Gastrointestinal hormone

The demand for plant-based proteins has been rapidly increasing due to sustainability, ethical and health reasons. present study aimed investigate the digestion characteristics of three plant (quinoa, barley mungbean) based on an in vitro model effect their simulated gastrointestinal digests satiety hormone cholecystokinin (CCK) secretion enteroendocrine STC-1 cells. nitrogen distribution process, relative molecular weight (MW) peptides amino acid composition were characterized. Quinoa protein had highest proportion soluble after (85.79%), followed by (74.98%) mungbean (64.14%), suggesting that quinoa was more easily digested than proteins. but not free acids main components quinoa, barley, digest a well balanced pattern, whereas lacking Lys, short sulfur (Phe + Tyr) rich Lys. In terms ability stimulate CCK secretion, strong stimulatory while only weak effect. After pretreatment with specific calcium-sensing receptor (CaSR) antagonist NPS 2143, induced greatly suppressed, indicating CaSR involved digest-induced secretion. These results show good nutritional quality, is excellent source potential application as dietary supplement obesity management.