DHA-enriched phosphatidylserine alleviates high fat diet-induced jejunum injury in mice by modulating gut microbiota

• DOI: 10.1039/d2fo03019e
• Publication Date: 2023-01-07T07:01:42Z
• Authors: Shanshan Tian; Yanfeng Zhao; Li Qian; Su Jiang; Yunping Tang; Tao Han
• Keywords: Jejunum; Occludin; Malondialdehyde

A long-term high-fat diet (HFD) is one of the high-risk factors for intestinal barrier damage. Docosahexaenoic acid-enriched phosphatidylserine (DHA-PS) has multiple biological activities, while its protective effect on HFD-caused jejunum injury remains unknown. Thus, present study investigated DHA-PS HFD-induced in mice. Our results showed that (100 mg per kg d) could protect against by decreasing levels inflammatory such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) serum tissues, with histological analysis confirming this amelioration. Additionally, alleviated oxidative stress malondialdehyde (MDA) increasing total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) catalase (CAT) jejunum. Moreover, significantly increased expression tight junction proteins (ZO-1, occludin, claudin-4) jejunum, modulated gut microbiota disorder Firmicutes Bacteroidetes ratio, reducing relative abundance Lachnoclostridium, Coriobacteriaceae, Desulfovibrionaceae, Helicobacter, Lachnospiraceae_NK4A136_group, Alistipes, norank_f__Muribaculaceae, Bacteroides. Overall, these support can alleviate injury.