Red raspberry supplementation mitigates alcohol-induced liver injury associated with gut microbiota alteration and intestinal barrier dysfunction in mice

• DOI: 10.1039/d2fo03245g
• Publication Date: 2022-12-05T13:01:01Z
• Authors: Daniel Zogona; Abel Wend-Soo Zongo; Abdeen E. Elkhedir; Mahmoud Salah; Mingfang Tao; Rong Li; Ting Wu; Xiaoyun Xu
• Keywords: Occludin; Malondialdehyde

Alcoholic liver disease (ALD) is still a global health concern. Long-term alcohol intake alters the gut microbiota diversity and metabolic activity, causes intestinal barrier dysfunction, leading to development of ALD. This research explored protective effects underlying mechanisms red raspberry (RR) on alcohol-related disorders in mice. Male C57BL/6J mice were fed standard diet or supplemented with 2%, 4%, 8% weight/weight RR. Meanwhile, administered 35% (v/v) ethanol (EtOH, 10 mL per kg body weight) intragastrically once daily for six weeks, except control group The results showed that RR supplementation decreased injury markers (alanine aspartate transaminases) serum, reduced triglyceride level downregulated hepatic cytochrome P450 2E1 mRNA expression EtOH. In addition, EtOH-mediated oxidative stress was attenuated by through malondialdehyde content increased antioxidant (glutathione, glutathione peroxidase, catalase) levels activities exposed Moreover, reversed EtOH-induced alteration cecal microbial composition at phylum, order, genus, species improved function associated inhibition NF-κB/MLCK pathway, which accompanied upregulation tight junctions (zonula occludens 1, occludin, claudin-1, claudin-4) E-cadherin protein expressions. Accordingly, resulted endotoxins serum attenuation inflammatory response liver, illustrated significant decrease tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 levels. Overall, alleviated adverse chronic could be potential supplement improving