Study onLactiplantibacillus plantarumR6-3 from Sayram Ketteki to prevent chronic unpredictable mild stress-induced depression in mice through the microbiota–gut–brain axis

• DOI: 10.1039/d2fo03708d
• Publication Date: 2023-03-06T12:41:07Z
• Authors: Lina Zhao; Dongyao Li; Bimal Chitrakar; Chen Li; Na Zhang; Shaogang Zhang; Xinyu Wang; Miaoshu Wang; Hongtao Tian; Yunbo Luo
• Keywords: Gut–brain axis; Depression; Chronic Stress

The prevention, mitigation and treatment of depression has become a global issue that needs to be solved urgently. Sayram Ketteki, traditional natural fermented yoghurt from the region with world's fourth highest life expectancy, been known as "longevity secret", whose longevity anti-depression factors are speculated come its rich microorganisms. Therefore, for first time, we systematically studied in depth microbes screened new edible probiotic strain, Lactiplantibacillus plantarum R6-3, explored effect chronic unpredictable mild stress (CUMS)-induced mice. It is encouraging L. R6-3 was significantly superior classic anti-depressant drug, fluoxetine, performance promoting sucrose preference test (SPT) behavior by 18% (p < 0.001), lowering serum CORT content 5.6% 0.05), accelerating brain-derived neurotrophic factor (BDNF) level 5.9% 0.01), increasing IL-10 concentration 2.3% up-regulating expression BDNF phosphorylated-ERK 74% 0.01) 45% respectively, facilitating secretion fecal short-chain fatty acids (SCFAs), including n-butyric, n-valeric, isovaleric acid 47% 42% 0.05) 38% respectively. Through microbiota-gut-brain axis, promoted intestinal SCFAs through regulation composition function gut microbiota, activated production monoamine neurotransmitter, renewed brain factor, suppressed hyperactivity hypothalamic-pituitary-adrenal (HPA) axis adjusting hippocampal BDNF/TrkB/ERK/CREB signaling pathway, thereby improving immune oxidative status, protecting tissue damage, maintaining healthy weight preventing CUMS-induced depressive great prospects development functional foods, prevention innovative microecological preparations.