Multiplexed molecular imaging with surface enhanced resonance Raman scattering nanoprobes reveals immunotherapy response in mice via multichannel image segmentation

• DOI: 10.1039/d2nh00331g
• Publication Date: 2022-10-20T12:22:00Z
• Authors: Chrysafis Andreou; Konstantinos Plakas; Naxhije Berisha; Mathieu Gigoux; Lauren E. Rosch; Rustin Mirsafavi; Anton Oseledchyk; Suchetan Pal; Dmitriy Zamarin; Taha Merghoub; Michael R. Detty; Moritz F. Kircher
• Keywords: Mice; 0303 health sciences; 03 medical and health sciences; Neoplasms; Tumor Microenvironment; Animals; Immunologic Factors; Immunotherapy; Spectrum Analysis, Raman; Antibodies; Molecular Imaging; 3. Good health

Multiplexed imaging of 8 molecular targets with SERS nanoprobes in mice reveals spatially heterogeneous tumor responses to immunotherapy. Multichannel image segmentation enables classification of the tumor regions into responders and naïve.