3D or not 3D: a guide to assess cell viability in 3D cell systems

• DOI: 10.1039/d2sm00018k
• Publication Date: 2022-02-15T10:23:06Z
• Authors: Polina Bikmulina; Nastasia Kosheleva; Yuri Efremov; Artem Antoshin; Zahra Heydari; Valentina Kapustina; Valery Royuk; Vasily Mikhaylov; Victor Fomin; Massoud Vosough; Peter Timashev; Yury Rochev; Anastasia Shpichka
• Keywords: Viability assay; Cell Survival; 3D cell culture

Cell viability is the primary integrative parameter used for various purposes, particularly when fabricating tissue equivalents (e.g., using bioprinting or scaffolding techniques), optimizing conditions to cultivate cells, testing chemicals, drugs, and biomaterials, etc. Most of conventional methods were originally designed a monolayer (2D) culture; however, 2D approaches fail adequately assess tissue-engineered construct's drug effects recapitulate host-pathogen interactions infectivity. This study aims at revealing influence particular 3D cell systems' parameters such as components' concentration, gel thickness, density, on applicability standard assays. Here, we present an approach achieving adequate reproducible results in collagen- fibrin-based systems Live/Dead, AlamarBlue, PicoGreen Our have demonstrated that routine precise analysis should be performed combination least three based different properties, e.g. metabolic activity, proliferative capacity, morphology,