A dual-mode “signal-on” split-type aptasensor for bisphenol A via target-induced hybridization chain reaction amplification

• DOI: 10.1039/d3an01586f
• Publication Date: 2023-10-25T09:01:44Z
• Authors: You Wu; Zheng Wang; Jing Li; Jingjing Yang; Yinzhuo Shen; Hongbo Li; Xiao-Ya Hu; Qin Xu
• Keywords: Aptamer; Chain reaction; Dual mode; Photocurrent; DNA–DNA hybridization; Linear range

Herein, a dual-mode detection system was constructed for efficient and accurate of bisphenol A (BPA) with the assistance BPA-induced hybridization chain reaction (HCR). The captured DNA (cDNA) first modified on surface magnetic spheres gold nanoparticles polydopamine then hybridized BPA aptamer to form double-stranded (dsDNA). In presence target, released from sphere. free cDNA triggered HCR construct duplex. Methylene blue (MB), as bifunctional probe, intercalated into amplify photocurrent (IPEC) CdS-modified electrode generate an electrochemical current (IEC) at same time. Under optimized conditions, PEC EC signal responses were linear logarithm concentration in range 1.0 × 10-10 M 10-5 M. limits found be 1.27 10-11 3.0 using methods, respectively. biosensor exhibited good performance real sample analysis, demonstrating its promising potential practical applications. addition, this strategy provides more reliable results.