Direct immunoassay on a polyester microwell plate for colorimetric detection of the spike protein in swab and saliva samples

• DOI: 10.1039/d3ay01755a
• Publication Date: 2023-11-21T10:55:07Z
• Authors: Nikaele S. Moreira; Thaisa A. Baldo; Lucas C. Duarte; Leonardo Lopes-Luz; Karoliny A. Oliveira; Paulo F. N. Estrela; Amanda M. Simões; Samira Bührer-Sékula; Gabriela R. M. Du...; Wendell K. T. Coltro
• Keywords: Immunoassay; Spike Glycoprotein, Coronavirus; Humans; COVID-19; Colorimetry; Saliva; 3. Good health

This study presents the development of a polyester microplate for detecting S-protein SARS-CoV-2 virus in saliva and nasopharyngeal swab samples using direct enzyme-linked immunosorbent assay (ELISA) technology. The was designed to contain 96 zones with 3 mm diameter each, volume 2-3 μL. experimental conditions including reagent concentration reaction time were optimized. image digitized analyzed graphical software. linear range obtained between protein S concentrations pixel intensity 0-10 μg mL