Lactobacillus paracasei ET-22 and derived postbiotics reduce halitosis and modulate oral microbiome dysregulation – a randomized, double-blind placebo-controlled clinical trial

• DOI: 10.1039/d3fo02271d
• Publication Date: 2023-07-12T01:42:44Z
• Authors: Guna Wuri; Fudong Liu; Zhe Sun; Bing Fang; Wen Zhao; Wei-Lian Hung; Wei-Hsien Liu; Xiaoxu Zhang; Ran Wang; Fang Wu; Liang Zhao; Ming Zhang
• Keywords: Dysbiosis; Lactobacillus paracasei; Peptostreptococcus

Oral microbial dysbiosis is the primary etiologic factor for halitosis and may be critical preventive target halitosis. This study included randomized controlled trials (RCTs) assessing effects of Lactobacillus paracasei ET-22 live heat-killed bacteria on related oral microbiome. 68 subjects were divided into placebo, (ET-22.L) (ET-22.HK) groups. Subjects took different lozenges three times a day 4 weeks underwent saliva collection assessment breath volatile sulfur compound (VSC) levels at beginning end intervention. Salivary organic compounds measured using HS-SPME-GC/MS, microbiome profile was determined by 16S rRNA gene amplicon sequencing. A positive decrease in observed means both ET-22.L ET-22.HK groups after intervention, being more marked group (p = 0.0148). Moreover, intervention remarkably changed composition total salivary (VOCs) aroma-active VOCs. Key undesirable VOCs, such as indole, pyridine, nonanoic acid, benzothiazole, valeric significantly reduced. Meanwhile, or also altered taxonomic The pathogens Rothia Streptococcus reduced pathogenic Solobacterium Peptostreptococcus inhibited group. Collectively, our suggests that can inhibit production odor with halitosis, which to changes