An integrative temperature-controlled microfluidic system for budding yeast heat shock response analysis at the single-cell level

• DOI: 10.1039/d4lc00313f
• Publication Date: 2024-06-18T00:41:22Z
• Authors: Jie Hong; Hao He; Yinjia Xu; Shujing Wang; Chunxiong Luo
• Keywords: Budding yeast; Budding

Cells can respond and adapt to complex forms of environmental change. Budding yeast is widely used as a model system for these stress response studies. In studies, the precise control environment with high temporal resolution most important. However, there lack single-cell research platforms that enable temperature form cell growth. This has hindered our understanding cellular coping strategies in face diverse Here, we developed novel temperature-controlled microfluidic platform integrates microheater (using liquid metal) thermocouple (liquid metal