Studying the small extracellular vesicle capture efficiency of magnetic beads coated with tannic acid

• DOI: 10.1039/d4tb00127c
• Publication Date: 2024-06-07T15:41:03Z
• Authors: Nikita A. Grishaev; Ekaterina O. Mois...; Vasiliy S. Cherny...; Aleksei S. Komlev; Anton M. Novoselov; Alexey M. Yashchenok
• Keywords: Tannic acid; Extracellular Vesicles

The isolation of small extracellular vesicles (sEVs), including those secreted by pathological cells, with high efficiency and purity is highly demanded for research studies practical applications. Conventional sEV methods suffer from low yield, presence contaminants, long-term operation costs. Bead-assisted platforms are considered to be effective trapping sEVs recovery yield sufficient further molecular profiling. In this study, magnetically responsive beads made calcium carbonate (CaCO3) particles impregnated iron oxide (Fe3O4) nanoparticles fabricated using a freezing-induced loading (FIL) method. developed magnetic demonstrate magnetization can collected permanent magnet, ensuring their rapid gentle capture an aqueous solution. tannic acid on the surface formed layer-by-layer (LbL) method used induce coupling beads. These coated (TAMB) were applied derived MCF7 HCT116 cell lines. Quantitative data nanoparticle tracking analysis (NTA) BCA revealed about 60%. High-resolution transmission electron microscopy (HRTEM) imaging TAMBs indicated structural integrity. Compared size exclusion chromatography (SEC) method, proposed approach demonstrated comparable in terms purity, while offering relatively short time. results highlight potential TAMB enrichment biological fluids, such as culture media.