A novel PEGylation of chitosan nanoparticles for gene delivery

• DOI: 10.1042/ba20060163
• Publication Date: 2007-03-16T13:19:19Z
• Authors: Yanqiong Zhang; Jiji Chen; Yangde Zhang; Yifeng Pan; Jingfeng Zhao; Lifeng Ren; Mingmei Liao; Zhiyuan Hu; Lu Kong; Jiwei Wang
• Keywords: PEGylation

CS (chitosan) has emerged as a promising non-viral vector for gene delivery because of its ability to form complexes with pDNA (plasmid DNA) and enhance transport across cellular membranes through endocytosis. Complexes may improve transfection efficiency; however, they are not capable sustained DNA release prolonging transfer. In order achieve prolonged CS-DNA complexes, we prepared NP (nanoparticle) complexes. alpha-Methoxy-omega-succinimidylpoly(ethylene glycol) was then conjugated the surface using an active ester scheme; finally, potential PEGylation [poly(ethylene glycol)ylation] gene-delivery transfer exogenous genes in vitro vivo were examined. Electrophoretic analysis suggested that NPs could protect from nuclease degradation. The carried by enter be expressed HepG2 cells. However, efficiency very low highest dose transferred 1.6 microg. activities CS-DNA-PEG preserved higher (2.4 microg) transferred. This indicated PEGylated had been improved. experiments also showed mediated expression tissues than did tumours induced all. These results improves or deliver therapeutic directly into hepatoma tissues.