A dinoflagellate CDK5‐like cyclin‐dependent kinase

• DOI: 10.1042/bc20070018
• Publication Date: 2007-08-28T15:37:04Z
• Authors: Thierry Bertomeu; Jean Rivoal; David Morse
• Keywords: Dinoflagellate; Polo-like kinase; Cyclin-dependent kinase 5

Background information . Mitosis during the dinoflagellate cell cycle is unusual in that nuclear envelope remains intact and segregation of permanently condensed chromosomes uses a cytoplasmic mitotic spindle. To examine regulation context these features, it necessary to isolate characterize regulators such as CDK (cyclin‐dependent kinase). Results We report characterization from Lingulodinium polyedrum This reacts with an anti‐PSTAIRE antibody was identified by protein microsequencing after partial purification. The microsequence shows homology toward Pho85/CDK5 clade CDKs. Neither amount nor phosphorylation state changed over course cycle, agreement results reported for CDK5 family members other systems. Conclusions conclude we have probably isolated CDK5‐like protein. data here support identification this homologue, suggest dinoflagellates may contain several families.