Hydrogenosomes in the rumen protozoon Dasytricha ruminantium Schuberg

• DOI: 10.1042/bj2000365
• Publication Date: 2015-08-10T20:32:47Z
• Authors: N Yarlett; A C Hann; D Lloyd; A Williams
• Keywords: Organelle; Malate dehydrogenase; Hydrogenase

This paper reports for the first time presence in anaerobic rumen ciliate Dasytricha ruminantium (Schuberg) of microbody-like organelles, about 0.5 micrometer diameter, with a granular matrix and an equilibrium density approx. 1.18 g/ml. These organelles can be isolated fraction sedimented at 10(5) g-min that contains 67% total pyruvate synthase (EC 1.2.7.1), 66% hydrogenase 1.18.3.1) 20% lactate dehydrogenase 1.1.1.27). Thus several respects this is enzymically similar to those containing hydrogenosomes some other parasitic protozoa (the trichomonads). However, contrast trichomonads, oxygen-tolerant enzyme malate (decarboxylating) 1.1.1.40) not particulate, but occurs only cytosol. results enable proposal scheme pathway product formation (acetate, lactate, CO2 H2) from carbohydrates.