Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities

• DOI: 10.1042/bj20040511
• Publication Date: 2004-08-10T08:40:56Z
• Authors: Scott J. ROBERTS; Alan J. STEWART; Peter J. SADLER; Colin FARQUHARSON
• Keywords: Phosphocholine

Human PHOSPHO1 is a phosphatase enzyme for which expression upregulated in mineralizing cells. This has been implicated the generation of Pi matrix mineralization, process central to skeletal development. member haloacid dehalogenase (HAD) superfamily Mg2+-dependent hydrolases. However, substrates are, as yet, unidentified and little known about its activity. We show here that exhibits high specific activities toward phosphoethanolamine (PEA) phosphocholine (PCho). Optimal enzymic activity was observed at approx. pH 6.7. The shows Mg2+-dependence, with apparent Km values 3.0 μM PEA 11.4 PCho. These results provide novel mechanism cells from