Human ornithine decarboxylase paralogue (ODCp) is an antizyme inhibitor but not an arginine decarboxylase

• DOI: 10.1042/bj20071004
• Publication Date: 2007-12-13T11:17:14Z
• Authors: Kristiina Kanerva; Laura T. Mäkitie; Anna Pelander; Marja Heiskala; Leif C. Andersson
• Keywords: Ornithine decarboxylase antizyme; Arginine decarboxylase

ODC (ornithine decarboxylase), the rate-limiting enzyme in polyamine biosynthesis, is regulated by specific inhibitors, AZs (antizymes), which turn are inhibited AZI (AZ inhibitor). We originally identified and cloned cDNA for a novel human ODC-like protein called ODCp (ODC paralogue). Since was devoid of catalytic activity, we proposed that form AZI. has subsequently been suggested to function either as mammalian ADC (arginine decarboxylase) or mice. Here, report (AZIN2). By using yeast two-hybrid screening vitro binding assay, show binds AZ1–3. Measurements activity degradation assay reveal inhibits AZ1 efficiently both vivo. further demonstrate ubiquitin-dependent AZ1-independent similar also no intrinsic activity.