Secret of the major birch pollen allergen Bet v 1: identification of the physiological ligand

• DOI: 10.1042/bj20130413
• Publication Date: 2013-10-31T10:02:16Z
• Authors: Christian Seutter...; Thomas Hoffmann; Maximilian J. Hartl; Kristian Schweimer; Wilfried Schwab; Paul Rösch; Olivia Hartl-Spie...
• Keywords: BET theory; Heteronuclear molecule

The major birch pollen allergen Bet v 1 is the main elicitor of airborne type I allergies and belongs to PR-10 family (pathogenesis-related proteins 10). most extensively studied allergen, well characterized at a biochemical immunological level; however, its physiological function remains elusive. In present study, we identify Q3OS (quercetin-3-O-sophoroside) as natural ligand 1. We isolated bound from mature confirmed binding by reconstitution 1-Q3OS complex. Fluorescence UV-visible spectroscopy experiments, HSQC (heteronuclear single-quantum coherence) titration, comparison with model compounds, such quercetin, indicated specificity binding. Elucidation site NMR combined computational resulted in more detailed understanding shed light on postulate that plays an important, but yet unclear, role during inflammation response recognition IgE.