Comparison of human stromelysin and collagenase by cloning and sequence analysis

• DOI: 10.1042/bj2400913
• Publication Date: 2015-08-10T20:57:04Z
• Authors: S E Whitham; G Murphy; P Angel; H J Rahmsdorf; B J Smith; A Lyons; T J R Harris; J J Reynolds; P Herrlich; A J P Docherty
• Keywords: Thermolysin; Cloning (programming); Homology

A comparison of the cDNA-derived amino acid sequences human stromelysin and collagenase with N-terminal purified enzymes reveals that these metalloproteinases are highly conserved they secreted as proenzymes. putative zinc-binding site was identified by its homology zinc-chelating sequence thermolysin. These permitted identification of: transin, a protein induced in rat fibroblasts either exposed to growth factors or transformed oncogenic viruses, homologue stromelysin, XHF1, after treatment tumourigenic agents, collagenase.