Catabolism of aggrecan, decorin and biglycan in tendon

• DOI: 10.1042/bj3500181
• Publication Date: 2015-08-10T22:12:07Z
• Authors: Sarah G. REES; Carl R. FLANNERY; Chris B. LITTLE; Clare E. HUGHES; Bruce CATERSON; Colin M. DENT
• Keywords: Biglycan; Aggrecan; Aggrecanase; Versican; Catabolism; Lumican

We have examined the catabolism of proteoglycans aggrecan, decorin and biglycan in fresh tendon samples explant cultures tissue from tensional compressed regions young mature bovine tendons. A panel well-characterized antibodies that recognize glycosaminoglycan or protein (linear neoepitope) sequences was used to detect proteoglycan degradation products were both retained within released into culture medium. In addition, a reverse-transcriptase-mediated PCR analysis examine mRNA expression patterns aggrecanases. The results this study indicate major role for aggrecanase(s) aggrecan tendon. also provides characterization epitopes associated with tendon, illustrating age-related changes isomers chondroitin sulphate disaccharides remain attached core linkage region after digestion chondroitinase ABC. Evidence rapid turnover small observed, indicating additional molecular pathways might compromise integrity collagen matrix potentially contribute dysfunction injury during disease.