SRY gene transferred by extracellular vesicles accelerates atherosclerosis by promotion of leucocyte adherence to endothelial cells

• DOI: 10.1042/cs20140826
• Publication Date: 2015-03-20T09:52:13Z
• Authors: Jin Cai; Weiwei Guan; Xiaorong Tan; Caiyu Chen; Liangpeng Li; Na Wang; Xue Zou; Faying Zhou; Jialiang Wang; Fang Pei; Xinjian Chen; Hao Luo; Xinquan Wang; Duofen He; Lin Zhou; Pedro A. Jose; Chunyu Zeng
• Keywords: Testis determining factor; Gene knockin; Knockout mouse; Extracellular vesicles

We set out to investigate whether and how SRY (sex-determining region, Y) DNAs in plasma EVs (extracellular vesicles) is involved the pathogenesis of atherosclerosis. PCR gene sequencing found fragment from male, but not female, patients; male patients with CAD (coronary artery disease) had a higher GCN (gene copy number) than healthy subjects. Additional studies that leucocytes, major source EVs, mRNA protein expression controls. After incubation SRY-transfected HEK (human embryonic kidney)-293 cells, monocytes (THP-1) HUVECs umbilical vein endothelial cells), which do endogenously express protein, were newly synthesized protein. This resulted an increase adherence factors CD11-a THP-1 cells ICAM-1 (intercellular adhesion molecule 1) HUVECs. EMSA showed increased promoter activity There was adherent after SRY-EVs. transferred have pathophysiological significance vivo; injection into ApoE−/− (apolipoprotein-knockout) mice accelerated The vascular may play important role atherosclerosis; this mechanism provides new approach understanding inheritable men.