Plasma long non-coding RNA, CoroMarker, a novel biomarker for diagnosis of coronary artery disease

• DOI: 10.1042/cs20150121
• Publication Date: 2015-07-23T02:09:23Z
• Authors: Yujia Yang; Yue Cai; Gengze Wu; Xinjian Chen; Yukai Liu; Xinquan Wang; Junyi Yu; Chuanwei Li; Xiongwen Chen; Pedro A. Jose; Lin Zhou; Chunyu Zeng
• Keywords: Male; 0303 health sciences; RNA, Untranslated; Coronary Artery Disease; Middle Aged; 3. Good health; 03 medical and health sciences; Risk Factors; Case-Control Studies; Humans; Biomarkers; Aged; Oligonucleotide Array Sequence Analysis

Long non-coding RNAs (lncRNAs) have been reported to be involved in the pathogenesis of cardiovascular disease (CVD), but whether circulating lncRNAs can serve as a coronary artery (CAD), biomarker is not known. The present study screened by microarray analysis plasma from CAD patients and control individuals found that 265 were differentially expressed. To find specific possible candidates, we used following criteria for 174 up-regulated lncRNAs: signal intensity ≥8, fold change >2.5 P<0.005. According these criteria, five intergenic identified. After validation quantitative PCR (qPCR), one lncRNA was excluded candidate list. remaining four independently validated another population 20 individuals. Receiver operating characteristic (ROC) curve showed AC100865.1 (referred CoroMarker) best lncRNAs. CoroMarker levels also stable plasma. predictive value further assessed larger cohort with 221 187 Using diagnostic model Fisher's taking risk factors into account, optimal sensitivity increased 68.29% 78.05%, whereas specificity decreased slightly 91.89% 86.49%. because it mainly extracellular vesicles (EVs), probably monocytes. We conclude stable, sensitive CAD.